Analysis of qPCR data using qbase+

Trainer Janick Mathys
BITS Courses TRAINING AT VIB

Goal

This training specifically focuses on the analysis of gene expression data using qbase+. During this training you will learn to:

  • create an experiment in qbase+
  • import run data
  • customize sample properties
  • select reference genes
  • assess the quality of your qPCR data
  • visualize and export the results

Summary

This hands-on training provides an introduction to the analysis of qPCR data using Biogazelle's qbase+ software. Although this software allows to do various types of analyses (gene expression, copy number analysis, miRNA profiling), we will focus in the training on gene expression analysis. qbase+ software is freely distributed by BITS to VIB scientists. Therefore the training will be limited to VIB scientists.

Prerequisites

This training is intended for people with basic knowledge of the principles underlying qPCR.

Schedule

See the TRAINING AT VIB website for a detailed schedule of this training.

Training material

Links

The installation guide and the user manual can be found on the BITS qbase+ software page

Scientific topics Gene expression data analysis, Gene expression, PCR experiment
Target audience Life Science Researchers, PhD students, post-docs