Metagenomics

Trainer Falk Hildebrand (EMBL)
BITS Courses TRAINING AT VIB

Goal

Processing and statistical analysis of 16S rRNA metagenomic experiments using R & Lotus pipeline. The following analytical steps from raw data to communicable results will be on the programme

  • Preparation of sequencing data, read filtering, data cleaning
  • Data normalization
  • Numerical ecology: Clustering, Ordination, Diversity, Modelling
  • Univariaten & Multivariate Statistics
  • Biological interpretation

Summary

This training will start with the presentation of a 16S pipeline (psbweb05.psb.ugent.be/lotus/) in a linux environment, using a minimal amount of linux commands. This will enable the preprocessing of the data going from raw reads to taxonomic tables and phylogenetic trees.

The 2nd part of the training will give an overview of numerical ecology and takes part entierly in R.

Prerequisites

The training is intended for people who have some experience with R. Linux usage will be part of the course, but very basic LinuX knowledge is sufficient for this course. BITS provides 15 laptops for their training sessions. Depending on the number of participants (max 20), it might be that you have to share the laptop with one other participant but you can also choose to bring your own laptop for this training session.

Schedule

See the TRAINING AT VIB website for a detailed schedule of this training.

Training material

Exercises available on the wiki page

Links

none

Scientific topics Metagenomics
Target audience Life Science Researchers, PhD students, post-docs, beginner bioinformaticians